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British Journal of Nutrition. 92(6):921-929, December 2004. Berrougui, Hicham 1; Alvarez de Sotomayor, Maria 2,*; Perez-Guerrero, Concepcion 2; Ettaib, Abdelkader 1; Hmamouchi, Mohamed 1; Marhuenda, Elisa 2; Herrera, Maria Dolores 2 Traditionally hand-pressed argan oil, obtained from Argania spinosa seeds, is eaten raw in south-west Morocco; its rich composition of tocopherols, MUFA and PUFA make a study of its actions on risk factors for CVD, such as hypertension, interesting. The effects of 7 weeks of treatment with argan oil (10 ml/kg) on the blood pressure and endothelial function of spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats were investigated. Systolic blood pressure and heart rate were measured every week by the tail-cuff method and endothelial function was assessed by carbachol (10-8 to 10-4 M)-induced relaxations of aortic rings and small mesenteric arteries pre-contracted with phenylephrine. Argan-oil administration reduced the mean blood pressure of SHR after the fifth week of treatment (P < 0[middle dot]05) and increased (P < 0[middle dot]01) the endothelial responses of arteries from SHR. |
The NO synthase inhibitor, L-N-[omega]-nitroarginine (3x10-5 M) revealed a greater participation of NO in the relaxant effect after the treatment. When cyclooxygenase (COX) was blocked with indomethacin (10-5 M), an involvement of COX products in the endothelium-dependent response was characterized. Enzyme immunoassay of thromboxane B2 showed a significant decrease (P < 0[middle dot]05) in the release of thromboxane A2 in both aorta and small mesenteric artery after argan-oil treatment of SHR. Experiments in the presence of the thromboxane A2-prostaglandin H2 receptor antagonist ICI 192,605 (10-5 M) confirmed this result. Results after incubation with the antioxidants superoxide dismutase and catalase suggested that a decreased oxidative stress might contribute to explain the beneficial effects of argan-oil treatment.
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